10+Restriction+Fragment+Polymorphism

Restriction Fragments Length Polymorphism (RFLPs)

Describe it: RFLPs are restriction fragment length polymorphisms, which are variations in the short base sequences where restriction enzymes can cut DNA. RFLP refers to a difference between two or more samples of homologous DNA molecules arising differing locations of restriction sites.

Analyze it : In RFLP analysis, the DNA sample is broken into pieces by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel eletrophoresis.

Apply it: One way that RFLPs are used is in a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can also be used in paternity cases or criminal cases to determine the source of a DNA sample. Another way is RFLPs can be used determine the disease status of an individual. And RFLPs can be used to measure recombination rates which can lead to a genetic map.

Synthesize: RFLPs remind me of scissors because scissors cut paper and RFLPs cut the DNA.

Argument: RFLPs are a good thing because they help with many things. Like in a criminal case and they need to figure out who did it! Also when they use it to determine the disease status of someone.



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Sources: book wiki.com users.rcn.com