02+Nucleic+Acid+Hybridization

Part 1-DNA Cloning/Recombinant DNA/Genetic Engineering

1. Describe: process in which the DNA of a gene is detected by its ability to base-pair with a complementary sequence on another nucleic acid molecule

2. Analyze: A nucleic acid probe is a complementary molecule that is a short, single-stranded nucleic acid that can either be DNA or RNA. It is needed because it is the complementary sequence on which the DNA can be detected by another nucleic acid molecule. If even a small part on the nucleotide sequence for a nucleotide is known, a complementary probe can be made for it. The probe will use hydrogen bonds to bond to the nucleotide sequence. It is then marked with the florescent tag so it can be tracked.

3. Apply: It can be used to allow us to track certain sequences of the nucleotides. It also will form into the cells. The bacteria cells can then be tracked easier because it would be easy to see the difference between the kinds of bacteria cells in the colonies. Once the cells with nucleic acid hybridization are found, it is then able to allow us to grow them specifically in a culture to easily isolate large amounts of the gene. Then, the cloned gene can be used as a probe to identify similar DNA, such as DNA from other species.

4.Synthesize: This reminds me of fireflies because they can light up under certain conditions. It also reminds me of when bodies have the blood drained from them and the dyes from the embalming process are put into the veins are arteries. It marks the veins are arteries so the body will preserve more. So in other words, the body gets tagged with these dyes that help it preserve itself. The nucleic acid hybridization uses the florescent tags to see where the cells are, so they are slightly similar.

5. Argue: I am for this because it helps us figure out what bacteria is transformed. It also lets us be in control of experiments and allows us to control the bacteria. It also allows us to know how the bacteria cells can clone. Knowing what gene is what, it allows us to do research and experiments on just that one topic, which can lead to many other great discoveries. We can find out what species can do what with their genes and that may help us understand evolution more.

Sources: AP Biology textbook, eighth edition

Nucleic Acid Hybridization