05+Gel+Electrophoresis

In gel electrophoresis scientists can separate the DNA (or RNA) or proteins on the basis of size, electrical charge, and other physical properties. The DNA moves from the negative to the positive side, because DNA is negative so it is attracted to the positive side. The smaller fragment moves farther because they’re smaller and and maneuver through the gel better than larger fragments.
 * __ Describe: __**

The different types of DNA are inserted into the wells on the negative end of the gel. (This process is rather difficult because you are doing it underwater.) Then the electric current is turned on and the DNA moves to the opposite side, the smaller fragments moving farther. The bands won’t show up until a banding dye is added and it fluoresces pink in ultraviolet light. It is then placed on a light table so one can see the bands better.
 * __ Analyze: __**

Scientists use this when they want to compare DNA from a crime scene or find out who the father of a child is. They can take samples of the DNA from blood or skin cells and compare it to the control DNA or what they are comparing it too. If the sample matches up to the control, they have their person.
 * __ Apply: __**

This makes me think of CSI because they’re always investigating crime scene investigations and need to know who the victim is or who committed the crime. If they find DNA they can take DNA from the victim wrong-doer and with gel electrophoresis they can compare the DNA. If the bands are in the same place as the control one, then they know who the person is.
 * __ Synthesize: __**

I will argue for this. I mean, the bad guy needs to be caught right? Granted they probably don’t do gel electrophoresis anymore, but still. It’s never hurt anyone. And also, it’s fun to do, its really interesting to see how the bands move and what they tell who’s DNA it belongs to or what disease it is.
 * __ Argue For Or Against: __**



Sources: AP Biology book, eighth edition Lab from Mr. Kromann's BioMedical class